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Announcement

Clinical specimens submitted on paper forms after January 1^st, 2024 will be subject to rejection per WAC 246-101-215 without prior approval. Clinical specimens shipped to the WAPHL must be ordered through WAPHL LWP Portal. *Environmental samples will still be accepted via paper requisition form.

Pass Through Request

Specimen Pass Through Form

Please review all CDC submission requirements, including proper patient identifiers before shipping specimens to PHL.

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Specimen collection and shipping instructions
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Pre-approval requirements
Testing methodologies and frequencies
Turnaround times and contact information

To ensure that specimens meet laboratory acceptance criteria, please review all appropriate content prior to specimen submission.

Name Sort descending	Updated	Required form	Specimen Collection and Submission Instructions	Pre-approval Requirement Procedure, if applicable	Methodology	Testing Frequency	Turnaround Time (business days)	Comments	Contact	Footnotes	Notifiable Conditions Info
Measles IgG, EIA Rubeola	05/05/2022	Requisition Form - Virology (webform)	Measles IgG, EIA	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Enzyme-linked Immunosorbent Assay (EIA)	As needed*	2-5 days**	Additional testing methodologies performed for measles: Measles IgM EIA, Measles RT-PCR.	Virology (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Measles IgM, EIA Rubeola	05/05/2022	Requisition Form - Virology (webform)	Measles IgM, EIA	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Enzyme-linked Immunosorbent Assay (EIA)	As needed*	2-5 days**	Additional testing methodologies performed for measles: Measles IgG EIA, Measles RT-PCR.	Virology (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (turnaround time of 24 hours). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Measles, RT-PCR Measles PCR	05/05/2022	Requisition Form - Virology (webform)	Measles RT-PCR Measles Specimen Shipping Guide	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR)	Within 24 hours of arrival*	1 day**	Additional testing methodologies performed for measles: Serology.	Virology (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (turnaround time of 24 hours). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Mumps IgG, EIA	05/05/2022	Requisition Form - Virology (webform)	Mumps IgG, EIA	Send sera for testing commercially unless otherwise instructed by Local Health Jurisdiction (LHJ) or Washington State Department of Health Communicable Disease Epidemiology (DOH CD-Epi). Pre-approval required for testing at PHL. Healthcare providers and laboratories may obtain pre-approval by contacting the LHJ for the patient's county of residence. If testing at PHL is approved, LHJ staff must then notify DOH CD-Epi at 206-418-5500.	Enzyme-linked Immunosorbent Assay (EIA)	As needed*	2-5 days**	Additional testing methodologies performed for mumps: Mumps IgM EIA, Mumps RT-PCR, Mumps culture	Virology Lab (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Mumps IgM, EIA	05/05/2022	Requisition Form - Virology (webform)	Mumps IgM, EIA	Send sera for testing commercially unless otherwise instructed by Local Health Jurisdiction (LHJ) or Washington State Department of Health Communicable Disease Epidemiology (DOH CD-Epi). Pre-approval required for testing at PHL. Healthcare providers and laboratories may obtain pre-approval by contacting the LHJ for the patient's county of residence. If testing at PHL is approved, LHJ staff must then notify DOH CD-Epi at 206-418-5500.	Enzyme-linked Immunosorbent Assay (EIA)	As needed*	2-5 days**	Additional testing methodologies performed for mumps: Mumps IgG EIA, Mumps RT-PCR, Mumps culture	Virology Lab (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Mumps, RT-PCR Mumps PCR	09/07/2018	Requisition Form - Virology (webform)	Mumps RT-PCR	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR)	Within 24 hours of arrival*	3 days**	Additional testing methodologies performed for mumps: culture and serology.	Virology Lab (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Neisseria species Identification Neisseria ID, GNDC	06/01/2022	Requisition Form - Microbiology (webform)	Neisseria species Identification	Pre-approval not required.	Isolates are identified using selective plating and biochemicals. Neisseria meningitidis isolates are serotyped and receive antibiotic sensitivity testing.	Daily, on regular business days	4-7 days	Neisseria meningitidis isolates should be from sterile sites only.	Special Bacteriology (206) 418-5452		Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Non-variola Orthopox Vaccinia, Monkey pox, Cow pox, Camel pox, Mpox	08/27/2024	Requisition Form - Bioterrorism (webform)	Non-variola Orthopox	Pre-approval required. Contact the Local Health Jurisdiction (LHJ) of the patient's county of residence and Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Real-Time Polymerase Chain Reaction (RT-PCR), cell culture	Daily, on regular business days*	8 hours**		Bioterrorism (206) 418-5562	* Stat testing is available 24/7 for critical situations ** Confirmation is performed at the Centers for Disease and Control (CDC)	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Norovirus, RT-PCR Norovirus PCR	06/27/2023	Requisition Form - Virology (webform)	Norovirus, RT-PCR	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) at 206-418-5500.	Real-Time Reverse Transcriptase Polymerase Chain Reaction (rRT-PCR)	Within 24 hours of arrival	2-5 days*	Individual cases are not reportable—only norovirus outbreaks suspected to be foodborne are reportable.	Molecular Laboratory (206) 418-5563	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day).	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Orthopox Vaccinia, Monkey pox, Cow pox, Camel pox, Mpox	08/27/2024	Requisition Form - Bioterrorism (webform)	Orthopox	Pre-approval required. Contact the Local Health Jurisdiction (LHJ) of the patient's county of residence and Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Real-Time Polymerase Chain Reaction (RT-PCR), cell culture	Daily, on regular business days*	8 hours**		Bioterrorism (206) 418-5562	* Stat testing is available 24/7 for critical situations ** Confirmation is performed at the Centers for Disease and Control (CDC)	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Paralytic Shellfish Poisoning PSP, Saxitoxin, Biotoxin, Marine Toxin	03/30/2021		Contact the Shellfish Program to schedule testing and to obtain required form. Collection and Submission Instructions Shellfish Toxin Testing (PDF) Instructional Video	Contact the Shellfish Program to schedule testing and to obtain required form.	Bioassay	Test is performed as needed	Within 24 hours of sample collection	Shellfish Related Illness: Questions concerning illness associated with eating shellfish should be directed either to the Local Health Jurisdiction or to the Office of Shellfish Program.	Marine Biotoxins Lab (206) 418-5441 Shellfish Program (360) 236-3328 Washington State Department of Health Shellfish Program	National Shellfish Sanitation Program	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Parasite - Blood Confirmation Malaria, Babesia sp., Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, Plasmodium ovale, Microfilariae, Trypanosomes, parasites, blood parasite, blood parasites	11/23/2022	Requisition Form - Microbiology (webform)	Parasite - Blood Confirmation	Pre-approval not required.	Standard blood smear examination. Blood may be required for Malaria PCR speciation.*	Daily, on regular business days	2-5 days	Suspected organism must be documented on requisition form. Travel history required.	Parasitology (206) 418-5469	* Specimen submission is for reference purposes only; Diagnostic testing must be performed by submitting laboratory. Full workup of thick and thin smears may not be performed.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Parasite - Gross Specimen Identification Arthropod ID; Ascaris lumbricoides; Dermacentor species; Diphyllobothrium latum; Ixodes species; Taenia species; Tapeworm; Tick ID; Trichuris trichiura; Worm ID, parasites	11/23/2022	Requisition Form - Microbiology (webform)	Parasite - Gross Specimen Identification	Specimens are only accepted from Healthcare Providers (HCP).	Macroscopic examination	Daily, on regular business days	2-5 days	Suspected organism must be documented on requisition form.	Parasitology (206) 418-5469		Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Parasite - Intestinal Confirmation Amebiasis; Ascaris lumbricoides; Balantidium coli; Blastocystis hominis; Dientamoeba fragilis; Chilomastix mesnili; Diphyllobothrium latum; Endolimax nana; Entamoeba histolytica; Entamoeba species; Giardia lamblia; Hookworm; Hymenolepis species; Iodamoeba buetschlii; Paragonimus; Schistosoma mansoni; Schistosoma japonicum; Strongyloides stercoralis; Taenia species; Trichuris trichiura, parasites, intestinal parasite, intestinal parasites	11/23/2022	Requisition Form - Microbiology (webform)	Parasite - Intestinal Confirmation	Pre-approval not required.	Standard Ova & Parasite (O&P) examination procedures may include wetmount or trichrome.*	Daily, on regular business days	2-5 days	Suspected organism must be documented on requisition form.	Parasitology (206) 418-5469	* Specimen submission is for reference purposes only; Diagnostic testing must be performed by submitting laboratory. Full O&P workup may not be performed depending on suspected organism.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Parasite - Intestinal Screen Amebiasis; Ascaris lumbricoides; Balantidium coli; Blastocystis hominis; Dientamoeba fragilis; Chilomastix mesnili; Diphyllobothrium latum; Endolimax nana; Entamoeba histolytica; Entamoeba species; Giardia lamblia; Hookworm; Hymenolepis species; Iodamoeba buetschlii; Paragonimus; Schistosoma mansoni; Schistosoma japonicum; Strongyloides stercoralis; Taenia species; Trichuris trichiura, parasites, intestinal parasite, intestinal parasites	11/23/2022	Requisition Form - Microbiology (webform)	Parasite - Intestinal Screen	Pre-approval required. Diagnostic Parasitology Services is provided only to the Local Health Jurisdictions (LHJ) and State Agencies. All other laboratories must obtain pre-approval from the Parasitology Laboratory before shipping (206) 418-5469.	Standard Ova & Parasite (O&P) examination procedures may include wetmount or trichrome.	Daily, on regular business days	2-5 days	Suspected organism must be documented on requisition form.	Parasitology (206) 418-5469		Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Parasite - Miscellaneous Paragonimus; Paragonimiasis; Schistosoma haematobium; Schistosomiasis; Strongyloides stercoralis; Strongyloidiasis, parasites	11/23/2022	Requisition Form - Microbiology (webform)	Parasite - Miscellaneous	Pre-approval required. Diagnostic Parasitology Services is provided only to the Local Health Jurisdictions (LHJ) and State Agencies. All other laboratories must obtain pre-approval from the Parasitology Laboratory before shipping (206) 418-5469.	Standard examination procedures, may include; concentration, centrifugation, wetmount, and stains as necessary.	Daily, on regular business days	2-5 days	Suspected organism must be documented on requisition form.	Parasitology (206) 418-5469		Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rabies, DFA Rabies	05/23/2024	Requisition Form - Rabies (webform)	Rabies, DFA	Pre-approval required. All submissions must be pre-approved by and sent through the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must obtain pre-approval from Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500 before shipping.	Direct Fluorescent Antibody (DFA)	Daily, on regular business days*	30 hours**	1) Please see detailed information at: Guidelines for the Submission of Specimens for Rabies Testing Rabies Specimen Packaging Procedure 2) Further testing to rule out non-specific fluorescence or to confirm a positive with low antigen distribution is performed by the Centers for Disease Control and Prevention (CDC).	Virology Lab (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (turnaround time of 24 hours). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
BioFire Respiratory Panel 2.1 Adenovirus, Coronavirus 229E, Coronavirus HKU1, Coronavirus NL63, Coronavirus OC43, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Human Metapneumovirus, Human Rhinovirus/Enterovirus, Influenza A and Influenza B, Parainfluenza 1, 2, 3, and 4, Respiratory Synctial Virus, Bordetella parapertussis, Bordetella pertussis, Chlamydia pneumoniae, and Mycoplasma pneumoniae.	04/15/2024	Requisition Form - Virology (webform)	BioFire TAT Respiratory Panel Guidance for Medical Examiners (PDF)	All cases from the Medical Examiner Offices will be tested without prior approval. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	The BioFire Respiratory 2.1 (RP2.1) Panel is an FDA aproved platform.	As needed	5 business days	Adenovirus typing is not performed at WA PHL. Testing will be forwarded to CDC upon request. Positive Influenza specimens will be typed, and forwarded to CDC if applicable.		* Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Ricin Toxin Ricinus communis, ricin	06/30/2022	Biothreat Environmental Sample Submission and Chain-of-Custody Form (file)	Ricin Toxin	Pre-approval required. Contact the FBI (First responders), the Local Health Jurisdiction (LHJ) of the patient's county of residence, and Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Time resolved fluorescence (TRF).	Stat processing 24/7 for critical situations	8 hours*		Bioterrorism (206) 418-5562	* Results for confirmation have an extended turnaround time (15 days). Some confirmation processes are performed at CDC.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rubella IgG, EIA	05/05/2022	Requisition Form - Virology (webform)	Rubella IgG, EIA	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Enzyme-linked Immunosorbent Assay (EIA)	As needed*	2-5 days**	Another rubella testing option is: Rubella IgM EIA	Virology Lab (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rubella IgM, EIA	05/05/2022	Requisition Form - Virology (webform)	Rubella IgM, EIA	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Enzyme-linked Immunosorbent Assay (EIA)	As needed*	2-5 days**	Another rubella testing option is: Rubella IgG EIA	Virology Lab (206) 418-5458	* Stat testing is provided with prior approval by Department of Health Communicable Disease Epidemiology (for results on next business day). ** Results for additional testing have an extended turnaround time.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rule Out Campylobacter species Campylobacteriosis, Campylobacter jejuni, Campylobacter coli, R/O Campylobacter, Rule-Out Campylobacter	01/15/2015	Requisition Form - Microbiology (webform)	R/O Campylobacter species	Pre-approval required. Healthcare providers (HCP) and laboratories must obtain pre-approval by contacting the Local Health Jurisdiction (LHJ) of the patient's county of residence. LHJ staff must then notify Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	All specimens will be screened on non-selective and selective media.	Daily, on regular business days	7-14 days*		Enteric Bacteriology (206) 418-5456	* Turnaround times may be extended when an isolate must be submitted to another reference laboratory (e.g. CDC) for further characterization. This test is for Epi investigations only.	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rule out Mpox (Clade Determination) Monkey Pox	08/29/2024	Requisition Form - Monkey Pox (webform)	Rule out Mpox (Clade determination)	Pre-approval required. Contact the Local Health Jurisdiction (LHJ) of the patient's county of residence and Washington State Department of Health (DOH) Office of Communicable Disease Epidemiology (CDE) 206-418-5500.	Real-Time Polymerase Chain Reaction (RT-PCR)	Daily, on regular business day	1 day from receipt		Bioterrorism (206) 418-5481	Confirmation may be performed at the Centers for Disease Control and Prevention (CDC)	Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rule Out Salmonella species Salmonella, Salmonellosis, Test of Cure, R/O Salmonella, Rule-Out Salmonella	05/31/2022	Requisition Form - Microbiology (webform)	R/O Salmonella species	Positive test must be shipped, no pre-approval necessary.	All specimens will be screened with selective plating. Suspicious isolates will have biochemical identification and serotyping performed.	Daily, on regular business days	7-14 days		Enteric Bacteriology (206) 418-5456		Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)
Rule Out Shiga Toxin-Producing E. coli (STEC) STEC, Toxin-Producing E. coli, Toxigenic E. coli, Test of cure, R/O Shiga Toxin-Producing E. coli, Rule-Out Shiga Toxin-Producing E. coli, R/O Shiga Toxigenic E. coli (STEC), Rule-Out Shiga Toxigenic E. coli (STEC), Rule Out STEC, Rule-Out STEC	05/31/2022	Requisition Form - Microbiology (webform)	R/O Shiga Toxigenic E. coli (STEC)	Positive tests must be shipped. No pre-approval necessary.	All specimens will have EIA-based shiga toxin test. Biochemical identification and serotyping will be performed on any shiga toxin positive isolates that grow on selective media.	Daily, on regular business days	7-14 days		Enteric Bacteriology (206) 418-5456		Notifiable Conditions Overview List of Notifiable Conditions (includes links to reporting forms, guidelines for public health investigations, and case definition)